› 3q0i

X-ray diffraction
1.89Å resolution

Methionyl-tRNA formyltransferase from Vibrio cholerae

Released:
DOI: 10.2210/pdb3q0i/pdb
PDB entry 3q0i coloured by chain, front view.
PDB entry 3q0i coloured by chain, side view.
PDB entry 3q0i coloured by chain, top view.
Source organism: Vibrio cholerae
Entry authors: Osipiuk J, Gu M, Papazisi L, Anderson WF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) = tetrahydrofolate + N-formylmethionyl-tRNA(fMet)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192147 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methionyl-tRNA formyltransferase Chain: A
Molecule details ›
Chain: A
Length: 318 amino acids
Theoretical weight: 34.84 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9KVU4 (Residues: 1-315; Coverage: 100%)
Gene names: VC_0045, fmt
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand GOL 1 x GOL
1 modified residue:
Ligand MSE 7 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P41212
Unit cell:
a: 66.692Å b: 66.692Å c: 183.782Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.176 0.203
Expression system: Escherichia coli BL21(DE3)

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