› 3q25

X-ray diffraction
1.9Å resolution

Crystal structure of human alpha-synuclein (1-19) fused to maltose binding protein (MBP)

Released:
DOI: 10.2210/pdb3q25/pdb
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PDB entry 3q25 coloured by chain, front view.
PDB entry 3q25 coloured by chain, side view.
PDB entry 3q25 coloured by chain, top view.
Source organisms:
Primary publication:
Structures of segments of α-synuclein fused to maltose-binding protein suggest intermediate states during amyloid formation.
Zhao M, Cascio D, Sawaya MR, Eisenberg D
Protein Sci 20 996-1004 (2011)
PMID: 21462277

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-142535 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Alpha-synuclein; Maltose/maltodextrin-binding periplasmic protein Chain: A
Molecule details ›
Chain: A
Length: 390 amino acids
Theoretical weight: 42.7 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEX9 (Residues: 27-392; Coverage: 99%)
  • Canonical: P37840 (Residues: 1-19; Coverage: 14%)
Gene names: JW3994, NACP, PARK1, SNCA, b4034, malE
Sequence domains: Bacterial extracellular solute-binding protein
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
2 bound ligands:
Ligand SO4 10 x SO4
Ligand GOL 6 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P43212
Unit cell:
a: 77.47Å b: 77.47Å c: 172.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.17 0.196
Expression system: Escherichia coli

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Citations

16 citation in other articles

A soluble α-synuclein construct forms a dynamic tetramer.
Wang et al. (2011)
15 more

106 mentions without citation

Genes and mutations causing retinitis pigmentosa.
Daiger et al. (2013)
105 more

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