3q3j

X-ray diffraction
1.97Å resolution

Crystal structure of plexin A2 RBD in complex with Rnd1

Released:
Source organism: Homo sapiens
Entry authors: Wang H, Tempel W, Tong Y, Guan X, Shen L, Buren L, Zhang N, Wernimont AK, Crombet L, Arrowsmith CH, Edwards AM, Bountra C, Weigelt J, Park H, Structural Genomics Consortium (SGC)

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-521878 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Plexin-A2 Chain: A
Molecule details ›
Chain: A
Length: 112 amino acids
Theoretical weight: 13.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O75051 (Residues: 1490-1600; Coverage: 6%)
Gene names: KIAA0463, OCT, PLXN2, PLXNA2, UNQ209/PRO235
Sequence domains: Plexin cytoplasmic RhoGTPase-binding domain
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
Rho-related GTP-binding protein Rho6 Chain: B
Molecule details ›
Chain: B
Length: 214 amino acids
Theoretical weight: 24.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q92730 (Residues: 5-200; Coverage: 85%)
Gene names: RHO6, RND1
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: I222
Unit cell:
a: 59.022Å b: 67.129Å c: 145.291Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.211 0.248
Expression system: Escherichia coli BL21