› 3q7c

X-ray diffraction
1.5Å resolution

Exonuclease domain of Lassa virus nucleoprotein bound to manganese

Released:
DOI: 10.2210/pdb3q7c/pdb
PDB entry 3q7c coloured by chain, front view.
PDB entry 3q7c coloured by chain, side view.
PDB entry 3q7c coloured by chain, top view.
Source organism: Lassa virus Josiah
Primary publication:
Structure of the Lassa virus nucleoprotein reveals a dsRNA-specific 3' to 5' exonuclease activity essential for immune suppression.
Hastie KM, Kimberlin CR, Zandonatti MA, MacRae IJ, Saphire EO
Proc Natl Acad Sci U S A 108 2396-401 (2011)
PMID: 21262835

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-537358 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nucleoprotein Chain: A
Molecule details ›
Chain: A
Length: 243 amino acids
Theoretical weight: 27.27 KDa
Source organism: Lassa virus Josiah
Expression system: Escherichia coli
UniProt:
  • Canonical: P13699 (Residues: 342-569; Coverage: 40%)
Gene name: N
Sequence domains: Arenavirus nucleocapsid C-terminal domain
Structure domains: Arenaviral nucleoprotein, C-terminal domain

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand MN 1 x MN
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 44.87Å b: 67.94Å c: 78.68Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.19 0.216
Expression system: Escherichia coli

Quick links

Citations

68 review citations

Nucleoproteins and nucleocapsids of negative-strand RNA viruses.
Ruigrok et al. (2011)
67 more

1 mention without citation

Structure of the Lassa virus nucleoprotein reveals a dsRNA-specific 3' to 5' exonuclease activity essential for immune suppression.
Hastie et al. (2011)