› 3q7x

X-ray diffraction
1.4Å resolution

Crystal structure of Symfoil-4P/PV1: de novo designed beta-trefoil architecture with symmetric primary structure, primitive version 1

Released:
DOI: 10.2210/pdb3q7x/pdb
PDB entry 3q7x coloured by chain, front view.
PDB entry 3q7x coloured by chain, side view.
PDB entry 3q7x coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Simplified protein design biased for prebiotic amino acids yields a foldable, halophilic protein.
Longo LM, Lee J, Blaber M
Proc Natl Acad Sci U S A 110 2135-9 (2013)
PMID: 23341608

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163919 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
de novo designed beta-trefoil architecture with symmetric primary structure Chain: A
Molecule details ›
Chain: A
Length: 132 amino acids
Theoretical weight: 14.53 KDa
Source organism: Synthetic construct
Expression system: Not provided
Structure domains: Trefoil (Acidic Fibroblast Growth Factor, subunit A)

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 46.725Å b: 48.666Å c: 67.599Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.182 0.205
Expression system: Not provided

Quick links

Citations

12 review citations

Protein adaptations in archaeal extremophiles.
Reed et al. (2013)
11 more

1 mention without citation

Simplified protein design biased for prebiotic amino acids yields a foldable, halophilic protein.
Longo et al. (2013)