› 3qdm

X-ray diffraction
2.8Å resolution

The complex between TCR DMF4 and human Class I MHC HLA-A2 with the bound MART-1(26-35)(A27L) decameric peptide

Released:
DOI: 10.2210/pdb3qdm/pdb
PDB entry 3qdm coloured by chain, front view.
PDB entry 3qdm coloured by chain, side view.
PDB entry 3qdm coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
TCRs used in cancer gene therapy cross-react with MART-1/Melan-A tumor antigens via distinct mechanisms.
Borbulevych OY, Santhanagopolan SM, Hossain M, Baker BM
J Immunol 187 2453-63 (2011)
PMID: 21795600

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-208590 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class I histocompatibility antigen, A alpha chain Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 31.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Melanoma antigen recognized by T-cells 1 Chain: C
Molecule details ›
Chain: C
Length: 10 amino acids
Theoretical weight: 985 Da
Source organism: Homo sapiens
Expression system: Not provided
T cell receptor alpha chain constant Chain: D
Molecule details ›
Chain: D
Length: 195 amino acids
Theoretical weight: 21.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
DMF4 beta chain Chain: E
Molecule details ›
Chain: E
Length: 242 amino acids
Theoretical weight: 27.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P212121
Unit cell:
a: 56.012Å b: 69.745Å c: 227.103Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.211 0.284
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

20 review citations

T cell antigen receptor recognition of antigen-presenting molecules.
Rossjohn et al. (2015)
19 more

10 mentions without citation

TCR Recognition of Peptide-MHC-I: Rule Makers and Breakers.
Szeto et al. (2020)
9 more