› 3qf7

X-ray diffraction
1.9Å resolution

The Mre11:Rad50 complex forms an ATP dependent molecular clamp in DNA double-strand break repair

Released:
DOI: 10.2210/pdb3qf7/pdb
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PDB entry 3qf7 coloured by chain, front view.
PDB entry 3qf7 coloured by chain, side view.
PDB entry 3qf7 coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
The Mre11:Rad50 structure shows an ATP-dependent molecular clamp in DNA double-strand break repair.
Lammens K, Bemeleit DJ, Möckel C, Clausing E, Schele A, Hartung S, Schiller CB, Lucas M, Angermüller C, Söding J, Strässer K, Hopfner KP
Cell 145 54-66 (2011)
PMID: 21458667

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-194846 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA double-strand break repair Rad50 ATPase Chains: A, B
Molecule details ›
Chains: A, B
Length: 365 amino acids
Theoretical weight: 41.07 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9X1X1 (Residues: 1-190, 686-852; Coverage: 42%)
Gene names: TM_1636, rad50
Sequence domains:
Structure domains:
DNA double-strand break repair protein Mre11 Chains: C, D
Molecule details ›
Chains: C, D
Length: 50 amino acids
Theoretical weight: 6.24 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9X1X0 (Residues: 343-385; Coverage: 11%)
Gene names: TM_1635, mre11
Sequence domains: Mre11 C-terminal domain, bacteria

Ligands and Environments

2 bound ligands:
Ligand ANP 2 x ANP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P1
Unit cell:
a: 49.149Å b: 68.4Å c: 71.111Å
α: 98.64° β: 111.14° γ: 92.03°
R-values:
R R work R free
0.158 0.157 0.191
Expression system: Escherichia coli

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Citations

49 review citations

Double-strand break end resection and repair pathway choice.
Symington et al. (2011)
48 more

7 mentions without citation

Turning the Mre11/Rad50 DNA repair complex on its head: lessons from SMC protein hinges, dynamic coiled-coil movements and DNA loop-extrusion?
Zabolotnaya et al. (2020)
6 more

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