› 3qh7

X-ray diffraction
2.5Å resolution

2.5 A resolution structure of Se-Met labeled CT296 from Chlamydia trachomatis

Released:
DOI: 10.2210/pdb3qh7/pdb
PDB entry 3qh7 coloured by chain, front view.
PDB entry 3qh7 coloured by chain, side view.
PDB entry 3qh7 coloured by chain, top view.
Source organism: Chlamydia trachomatis 434/Bu
Primary publication:
Ab initio structural modeling of and experimental validation for Chlamydia trachomatis protein CT296 reveal structural similarity to Fe(II) 2-oxoglutarate-dependent enzymes.
Kemege KE, Hickey JM, Lovell S, Battaile KP, Zhang Y, Hefty PS
J Bacteriol 193 6517-28 (2011)
PMID: 21965559

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-102146 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CT296 Chain: A
Molecule details ›
Chain: A
Length: 157 amino acids
Theoretical weight: 18.22 KDa
Source organism: Chlamydia trachomatis 434/Bu
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H3MBY2 (Residues: 1-155; Coverage: 100%)
Gene name: CTL0548
Sequence domains: Domain of unknown function (DUF5070)
Structure domains: Domain of unknown function (DUF5070)

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P21212
Unit cell:
a: 50.827Å b: 63.663Å c: 46.32Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.203 0.258
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

The alternative translational profile that underlies the immune-evasive state of persistence in Chlamydiaceae exploits differential tryptophan contents of the protein repertoire.
Lo et al. (2012)
2 more

1 mention without citation

Ab initio structural modeling of and experimental validation for Chlamydia trachomatis protein CT296 reveal structural similarity to Fe(II) 2-oxoglutarate-dependent enzymes.
Kemege et al. (2011)