› 3qnq

X-ray diffraction
3.3Å resolution

Crystal structure of the transporter ChbC, the IIC component from the N,N'-diacetylchitobiose-specific phosphotransferase system

Released:
DOI: 10.2210/pdb3qnq/pdb
PDB entry 3qnq coloured by chain, front view.
PDB entry 3qnq coloured by chain, side view.
PDB entry 3qnq coloured by chain, top view.
Source organism: Bacillus cereus ATCC 10987
Primary publication:
Crystal structure of a phosphorylation-coupled saccharide transporter.
Cao Y, Jin X, Levin EJ, Huang H, Zong Y, Quick M, Weng J, Pan Y, Love J, Punta M, Rost B, Hendrickson WA, Javitch JA, Rajashankar KR, Zhou M
Nature 473 50-4 (2011)
PMID: 21471968

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180921 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Permease IIC component Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 442 amino acids
Theoretical weight: 48.11 KDa
Source organism: Bacillus cereus ATCC 10987
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q72XQ0 (Residues: 1-433; Coverage: 100%)
Gene names: BCE_5325, celB
Sequence domains: Phosphotransferase system, EIIC

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
2 bound ligands:
Ligand ZDM 4 x ZDM
Ligand CIT 7 x CIT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P43212
Unit cell:
a: 132.812Å b: 132.812Å c: 452.954Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.228 0.264
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

The bacterial phosphoenolpyruvate:carbohydrate phosphotransferase system: regulation by protein phosphorylation and phosphorylation-dependent protein-protein interactions.
Deutscher et al. (2014)
11 more

5 mentions without citation

Influences of membrane mimetic environments on membrane protein structures.
Zhou et al. (2013)
4 more