› 3qr6

X-ray diffraction
1.78Å resolution

Crystal Structure Analysis of H185F Mutant of Human CLIC1

Released:
DOI: 10.2210/pdb3qr6/pdb
Model geometry
Fit model/data
PDB entry 3qr6 coloured by chain, front view.
PDB entry 3qr6 coloured by chain, side view.
PDB entry 3qr6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Role of individual histidines in the pH-dependent global stability of human chloride intracellular channel 1.
Achilonu I, Fanucchi S, Cross M, Fernandes M, Dirr HW
Biochemistry 51 995-1004 (2012)
PMID: 22242893

Function and Biology Details

Reaction catalysed: 
2 glutathione + dehydroascorbate = glutathione disulfide + ascorbate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126230 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chloride intracellular channel protein 1 Chain: A
Molecule details ›
Chain: A
Length: 241 amino acids
Theoretical weight: 26.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O00299 (Residues: 1-241; Coverage: 100%)
Gene names: CLIC1, G6, NCC27
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P21
Unit cell:
a: 35.292Å b: 82.081Å c: 41.581Å
α: 90° β: 95.84° γ: 90°
R-values:
R R work R free
0.205 0.202 0.257
Expression system: Escherichia coli

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Citations

2 review citations

CLIC proteins, ezrin, radixin, moesin and the coupling of membranes to the actin cytoskeleton: a smoking gun?
Jiang et al. (2014)
1 more