› 3qtt

X-ray diffraction
2.6Å resolution

Crystal Structure of Pantoate-beta-alanine Ligase from Francisella tularensis Complexed with Beta-gamma ATP and Beta-alanine

Released:
DOI: 10.2210/pdb3qtt/pdb
PDB entry 3qtt coloured by chain, front view.
PDB entry 3qtt coloured by chain, side view.
PDB entry 3qtt coloured by chain, top view.
Source organism: Francisella tularensis subsp. tularensis
Entry authors: Maltseva N, Kim Y, Kwon K, Anderson WF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
ATP + (R)-pantoate + beta-alanine = AMP + diphosphate + (R)-pantothenate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177655 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pantothenate synthetase Chains: A, B
Molecule details ›
Chains: A, B
Length: 264 amino acids
Theoretical weight: 30.21 KDa
Source organism: Francisella tularensis subsp. tularensis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q5NF57 (Residues: 1-261; Coverage: 100%)
Gene names: FTT_1390, panC
Sequence domains: Pantoate-beta-alanine ligase
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ANP 2 x ANP
Ligand PRO 2 x PRO
Ligand MG 1 x MG
Ligand BAL 2 x BAL
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P43
Unit cell:
a: 47.147Å b: 47.147Å c: 257.661Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.168 0.251
Expression system: Escherichia coli BL21

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