› 3qui

X-ray diffraction
1.93Å resolution

Crystal structure of Pseudomonas aeruginosa Hfq in complex with ADPNP

Released:
DOI: 10.2210/pdb3qui/pdb
PDB entry 3qui coloured by chain, front view.
PDB entry 3qui coloured by chain, side view.
PDB entry 3qui coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Hfq binds ribonucleotides in three different RNA-binding sites.
Murina V, Lekontseva N, Nikulin A
Acta Crystallogr D Biol Crystallogr 69 1504-13 (2013)
PMID: 23897473

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-574738 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-binding protein Hfq Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 82 amino acids
Theoretical weight: 9.11 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HUM0 (Residues: 1-82; Coverage: 100%)
Gene names: PA4944, hfq
Sequence domains: Hfq protein
Structure domains: SH3 type barrels.

Ligands and Environments

4 bound ligands:
Ligand ADP 3 x ADP
Ligand ANP 3 x ANP
Ligand PEG 1 x PEG
Ligand ADE 1 x ADE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P212121
Unit cell:
a: 61.083Å b: 75.862Å c: 93.437Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.213 0.287
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Hfq: the flexible RNA matchmaker.
Updegrove et al. (2016)
3 more

3 mentions without citation

Structural analysis of full-length Hfq from Escherichia coli.
Beich-Frandsen et al. (2011)
2 more