› 3qzx

X-ray diffraction
1.3Å resolution

3D Structure of ferric methanosarcina acetivorans protoglobin Y61A mutant with unknown ligand

Released:
DOI: 10.2210/pdb3qzx/pdb
PDB entry 3qzx coloured by chain, front view.
PDB entry 3qzx coloured by chain, side view.
PDB entry 3qzx coloured by chain, top view.
Source organism: Methanosarcina acetivorans
Primary publication:
Structural heterogeneity and ligand gating in ferric Methanosarcina acetivorans protoglobin mutants.
Pesce A, Tilleman L, Dewilde S, Ascenzi P, Coletta M, Ciaccio C, Bruno S, Moens L, Bolognesi M, Nardini M
IUBMB Life 63 287-94 (2011)
PMID: 21618401

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186286 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Globin-sensor domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 195 amino acids
Theoretical weight: 22.92 KDa
Source organism: Methanosarcina acetivorans
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8TLY9 (Residues: 1-195; Coverage: 100%)
Gene name: MA_2883
Sequence domains: Protoglobin
Structure domains: Globins

Ligands and Environments

3 bound ligands:
Ligand HEM 1 x HEM
Ligand PO4 2 x PO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2
Unit cell:
a: 80.089Å b: 49.239Å c: 51.514Å
α: 90° β: 92.57° γ: 90°
R-values:
R R work R free
0.134 0.132 0.164
Expression system: Escherichia coli

Quick links

Citations

6 citation in other articles

Structure and haem-distal site plasticity in Methanosarcina acetivorans protoglobin.
Pesce et al. (2013)
5 more

1 mention without citation

Prediction of Protein Function from Tertiary Structure of the Active Site in Heme Proteins by Convolutional Neural Network.
Kondo et al. (2023)