› 3r09

X-ray diffraction
2.2Å resolution

Crystal structure of probable HAD family hydrolase from Pseudomonas fluorescens Pf-5 with bound Mg

Released:
DOI: 10.2210/pdb3r09/pdb
PDB entry 3r09 coloured by chain, front view.
PDB entry 3r09 coloured by chain, side view.
PDB entry 3r09 coloured by chain, top view.
Source organism: Pseudomonas protegens Pf-5
Entry authors: Vetting MW, Patskovsky Y, Toro R, Freeman J, Miller S, Sauder JM, Burley SK, Dunaway-Mariano D, Allen KN, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI), New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175599 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HAD-superfamily hydrolase, subfamily IA, variant 1 and 3 Chain: A
Molecule details ›
Chain: A
Length: 205 amino acids
Theoretical weight: 22.77 KDa
Source organism: Pseudomonas protegens Pf-5
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q4K5L5 (Residues: 1-197; Coverage: 100%)
Gene name: PFL_5400
Sequence domains: haloacid dehalogenase-like hydrolase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand SO4 1 x SO4
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P6122
Unit cell:
a: 66.299Å b: 66.299Å c: 251.29Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.181 0.179 0.208
Expression system: Escherichia coli BL21(DE3)

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