› 3r1j

X-ray diffraction
2.05Å resolution

Crystal structure of Alpha-ketoglutarate-dependent taurine dioxygenase from Mycobacterium avium, native form

Released:
DOI: 10.2210/pdb3r1j/pdb
PDB entry 3r1j coloured by chain, front view.
PDB entry 3r1j coloured by chain, side view.
PDB entry 3r1j coloured by chain, top view.
Source organism: Mycobacterium avium 104
Primary publication:
Increasing the structural coverage of tuberculosis drug targets.
Baugh L, Phan I, Begley DW, Clifton MC, Armour B, Dranow DM, Taylor BM, Muruthi MM, Abendroth J, Fairman JW, Fox D, Dieterich SH, Staker BL, Gardberg AS, Choi R, Hewitt SN, Napuli AJ, Myers J, Barrett LK, Zhang Y, Ferrell M, Mundt E, Thompkins K, Tran N, Lyons-Abbott S, Abramov A, Sekar A, Serbzhinskiy D, Lorimer D, Buchko GW, Stacy R, Stewart LJ, Edwards TE, Van Voorhis WC, Myler PJ
Tuberculosis (Edinb) 95 142-8 (2015)
PMID: 25613812

Function and Biology Details

Reaction catalysed: 
Taurine + 2-oxoglutarate + O(2) = sulfite + aminoacetaldehyde + succinate + CO(2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101737 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
TauD/TfdA-like domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 301 amino acids
Theoretical weight: 33.68 KDa
Source organism: Mycobacterium avium 104
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H2ZSG9 (Residues: 1-297; Coverage: 100%)
Gene name: MAV_4353
Sequence domains: Taurine catabolism dioxygenase TauD, TfdA family
Structure domains: Clavaminate synthase-like

Ligands and Environments

3 bound ligands:
Ligand EDO 3 x EDO
Ligand FE 2 x FE
Ligand CL 3 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: P21212
Unit cell:
a: 70.95Å b: 105.31Å c: 89.25Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.171 0.2
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Pleiotropic Functions of Tumor Suppressor WWOX in Normal and Cancer Cells.
Abu-Remaileh et al. (2015)
5 more