› 3r2c

X-ray diffraction
1.9Å resolution

Crystal Structure of Antitermination Factors NusB and NusE in complex with BoxA RNA

Released:
DOI: 10.2210/pdb3r2c/pdb
PDB entry 3r2c coloured by chain, front view.
PDB entry 3r2c coloured by chain, side view.
PDB entry 3r2c coloured by chain, top view.
Source organism: Aquifex aeolicus
Primary publication:
Structural basis for RNA recognition by NusB and NusE in the initiation of transcription antitermination.
Stagno JR, Altieri AS, Bubunenko M, Tarasov SG, Li J, Court DL, Byrd RA, Ji X
OpenAccess logo Nucleic Acids Res 39 7803-15 (2011)
PMID: 21652641

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-521365 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
Transcription antitermination protein NusB Chains: A, B
Molecule details ›
Chains: A, B
Length: 148 amino acids
Theoretical weight: 17.14 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O66530 (Residues: 1-148; Coverage: 100%)
Gene names: aq_133, nusB
Sequence domains: NusB family
Structure domains: NusB-like
Small ribosomal subunit protein uS10 Chains: J, K
Molecule details ›
Chains: J, K
Length: 83 amino acids
Theoretical weight: 9.5 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O66430 (Residues: 1-104; Coverage: 80%)
Gene names: aq_008, rpsJ
Sequence domains: Ribosomal protein S10p/S20e
Structure domains: Ribosomal protein S10 domain
5'-R(*GP*GP*CP*UP*CP*CP*UP*UP*GP*GP*CP*A)-3' Chains: R, S
Molecule details ›
Chains: R, S
Length: 12 nucleotides
Theoretical weight: 3.8 KDa

Ligands and Environments

2 bound ligands:
Ligand PEG 10 x PEG
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P21
Unit cell:
a: 40.53Å b: 113.008Å c: 65.763Å
α: 90° β: 102.87° γ: 90°
R-values:
R R work R free
0.171 0.169 0.202
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Bacteriophage lambda: Early pioneer and still relevant.
Casjens et al. (2015)
4 more

10 mentions without citation

Bacterial Transcription as a Target for Antibacterial Drug Development.
Ma et al. (2016)
9 more