› 3r9m

X-ray diffraction
1.95Å resolution

Crystal structure of the Brox Bro1 domain

Released:
DOI: 10.2210/pdb3r9m/pdb
PDB entry 3r9m coloured by chain, front view.
PDB entry 3r9m coloured by chain, side view.
PDB entry 3r9m coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The Phe105 loop of Alix Bro1 domain plays a key role in HIV-1 release.
Sette P, Mu R, Dussupt V, Jiang J, Snyder G, Smith P, Xiao TS, Bouamr F
Structure 19 1485-95 (2011)
PMID: 21889351

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178443 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
BRO1 domain-containing protein BROX Chain: A
Molecule details ›
Chain: A
Length: 376 amino acids
Theoretical weight: 42.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5VW32 (Residues: 2-374; Coverage: 91%)
Gene names: BROFTI, BROX, C1orf58
Sequence domains: BRO1-like domain
Structure domains: alix/aip1 like domains

Ligands and Environments

2 bound ligands:
Ligand EDO 8 x EDO
Ligand FMT 11 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P63
Unit cell:
a: 189.204Å b: 189.204Å c: 49.523Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.149 0.149 0.167
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Membrane fission reactions of the mammalian ESCRT pathway.
McCullough et al. (2013)
1 more

4 mentions without citation

Budding of a Retrovirus: Some Assemblies Required.
Rose et al. (2020)
3 more