› 3r9u

X-ray diffraction
2.36Å resolution

Thioredoxin-disulfide reductase from Campylobacter jejuni.

Released:
DOI: 10.2210/pdb3r9u/pdb
PDB entry 3r9u coloured by chain, front view.
PDB entry 3r9u coloured by chain, side view.
PDB entry 3r9u coloured by chain, top view.
Source organism: Campylobacter jejuni
Entry authors: Osipiuk J, Zhou M, Kwon K, Anderson KF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
Thioredoxin + NADP(+) = thioredoxin disulfide + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171066 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thioredoxin reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 315 amino acids
Theoretical weight: 33.94 KDa
Source organism: Campylobacter jejuni
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q0PBZ1 (Residues: 1-312; Coverage: 100%)
Gene names: Cj0146c, trxB
Sequence domains: Pyridine nucleotide-disulphide oxidoreductase
Structure domains: FAD/NAD(P)-binding domain

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
1 bound ligand:
Ligand EDO 1 x EDO
1 modified residue:
Ligand MSE 22 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C222
Unit cell:
a: 154.006Å b: 163.571Å c: 66.876Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.193 0.247
Expression system: Escherichia coli BL21(DE3)

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