› 3rq9

X-ray diffraction
1Å resolution

Structure of Tsi2, a Tse2-immunity protein from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb3rq9/pdb
PDB entry 3rq9 coloured by chain, front view.
PDB entry 3rq9 coloured by chain, side view.
PDB entry 3rq9 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Structural basis for type VI secretion effector recognition by a cognate immunity protein.
Li M, Le Trong I, Carl MA, Larson ET, Chou S, De Leon JA, Dove SL, Stenkamp RE, Mougous JD
OpenAccess logo PLoS Pathog 8 e1002613 (2012)
PMID: 22511866

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191231 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Immune protein Tsi2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 85 amino acids
Theoretical weight: 9.6 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9I0D9 (Residues: 1-77; Coverage: 100%)
Gene names: PA2703, tsi2
Sequence domains: T6SS, Immune protein Tsi2-like
Structure domains: Helix Hairpins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P1
Unit cell:
a: 27.807Å b: 34.063Å c: 37.65Å
α: 110.48° β: 95.94° γ: 95.82°
R-values:
R R work R free
0.147 0.145 0.176
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Type VI secretion system effectors: poisons with a purpose.
Russell et al. (2014)
9 more

7 mentions without citation

Molecular weaponry: diverse effectors delivered by the Type VI secretion system.
Alcoforado Diniz et al. (2015)
6 more