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Acoustically mounted microcrystals yield high-resolution X-ray structures.

Soares AS, Engel MA, Stearns R, Datwani S, Olechno J, Ellson R, Skinner JM, Allaire M, Orville AM
Biochemistry 50 4399-401 (2011)
Cited: 22 times
EuropePMC logo PMID: 21542590

Abstract

We demonstrate a general strategy for determining structures from showers of microcrystals. It uses acoustic droplet ejection to transfer 2.5 nL droplets from the surface of microcrystal slurries, through the air, onto mounting micromesh pins. Individual microcrystals are located by raster-scanning a several-micrometer X-ray beam across the cryocooled micromeshes. X-ray diffraction data sets merged from several micrometer-sized crystals are used to determine 1.8 Ǻ resolution crystal structures.

Articles - 3rto mentioned but not cited (4)

  1. Insulin analog with additional disulfide bond has increased stability and preserved activity. Vinther TN, Norrman M, Ribel U, Huus K, Schlein M, Steensgaard DB, Pedersen TÅ, Pettersson I, Ludvigsen S, Kjeldsen T, Jensen KJ, Hubálek F. Protein Sci 22 296-305 (2013)
  2. Characterization of Triticum aestivum Abscisic Acid Receptors and a Possible Role for These in Mediating Fusairum Head Blight Susceptibility in Wheat. Gordon CS, Rajagopalan N, Risseeuw EP, Surpin M, Ball FJ, Barber CJ, Buhrow LM, Clark SM, Page JE, Todd CD, Abrams SR, Loewen MC. PLoS One 11 e0164996 (2016)
  3. Acoustically mounted microcrystals yield high-resolution X-ray structures. Soares AS, Engel MA, Stearns R, Datwani S, Olechno J, Ellson R, Skinner JM, Allaire M, Orville AM. Biochemistry 50 4399-4401 (2011)
  4. Solvent minimization induces preferential orientation and crystal clustering in serial micro-crystallography on micro-meshes, in situ plates and on a movable crystal conveyor belt. Soares AS, Mullen JD, Parekh RM, McCarthy GS, Roessler CG, Jackimowicz R, Skinner JM, Orville AM, Allaire M, Sweet RM. J Synchrotron Radiat 21 1231-1239 (2014)


Reviews citing this publication (4)

  1. Serial femtosecond crystallography: A revolution in structural biology. Martin-Garcia JM, Conrad CE, Coe J, Roy-Chowdhury S, Fromme P. Arch Biochem Biophys 602 32-47 (2016)
  2. Protein crystallization screens developed at the MRC Laboratory of Molecular Biology. Gorrec F. Drug Discov Today 21 819-825 (2016)
  3. Dynamic Structural Biology Experiments at XFEL or Synchrotron Sources. Aller P, Orville AM. Methods Mol Biol 2305 203-228 (2021)
  4. Prospects of formamide as nitrogen source in biotechnological production processes. Schwardmann LS, Benninghaus L, Lindner SN, Wendisch VF. Appl Microbiol Biotechnol 108 105 (2024)

Articles citing this publication (14)

  1. Acoustic Injectors for Drop-On-Demand Serial Femtosecond Crystallography. Roessler CG, Agarwal R, Allaire M, Alonso-Mori R, Andi B, Bachega JFR, Bommer M, Brewster AS, Browne MC, Chatterjee R, Cho E, Cohen AE, Cowan M, Datwani S, Davidson VL, Defever J, Eaton B, Ellson R, Feng Y, Ghislain LP, Glownia JM, Han G, Hattne J, Hellmich J, Héroux A, Ibrahim M, Kern J, Kuczewski A, Lemke HT, Liu P, Majlof L, McClintock WM, Myers S, Nelsen S, Olechno J, Orville AM, Sauter NK, Soares AS, Soltis SM, Song H, Stearns RG, Tran R, Tsai Y, Uervirojnangkoorn M, Wilmot CM, Yachandra V, Yano J, Yukl ET, Zhu D, Zouni A. Structure 24 631-640 (2016)
  2. Precise Manipulation and Patterning of Protein Crystals for Macromolecular Crystallography Using Surface Acoustic Waves. Guo F, Zhou W, Li P, Mao Z, Yennawar NH, French JB, Huang TJ. Small 11 2733-2737 (2015)
  3. High-throughput in situ X-ray screening of and data collection from protein crystals at room temperature and under cryogenic conditions. Broecker J, Morizumi T, Ou WL, Klingel V, Kuo A, Kissick DJ, Ishchenko A, Lee MY, Xu S, Makarov O, Cherezov V, Ogata CM, Ernst OP. Nat Protoc 13 260-292 (2018)
  4. Ultrasonic acoustic levitation for fast frame rate X-ray protein crystallography at room temperature. Tsujino S, Tomizaki T. Sci Rep 6 25558 (2016)
  5. Technologies That Enable Accurate and Precise Nano- to Milliliter-Scale Liquid Dispensing of Aqueous Reagents Using Acoustic Droplet Ejection. Sackmann EK, Majlof L, Hahn-Windgassen A, Eaton B, Bandzava T, Daulton J, Vandenbroucke A, Mock M, Stearns RG, Hinkson S, Datwani SS. J Lab Autom 21 166-177 (2016)
  6. Nanolitre-scale crystallization using acoustic liquid-transfer technology. Villaseñor AG, Wong A, Shao A, Garg A, Donohue TJ, Kuglstatter A, Harris SF. Acta Crystallogr D Biol Crystallogr 68 893-900 (2012)
  7. High throughput screening using acoustic droplet ejection to combine protein crystals and chemical libraries on crystallization plates at high density. Teplitsky E, Joshi K, Ericson DL, Scalia A, Mullen JD, Sweet RM, Soares AS. J Struct Biol 191 49-58 (2015)
  8. A Versatile System for High-Throughput In Situ X-ray Screening and Data Collection of Soluble and Membrane-Protein Crystals. Broecker J, Klingel V, Ou WL, Balo AR, Kissick DJ, Ogata CM, Kuo A, Ernst OP. Cryst Growth Des 16 6318-6326 (2016)
  9. A linear relationship between crystal size and fragment binding time observed crystallographically: implications for fragment library screening using acoustic droplet ejection. Cole K, Roessler CG, Mulé EA, Benson-Xu EJ, Mullen JD, Le BA, Tieman AM, Birone C, Brown M, Hernandez J, Neff S, Williams D, Allaire M, Orville AM, Sweet RM, Soares AS. PLoS One 9 e101036 (2014)
  10. Developments in the Implementation of Acoustic Droplet Ejection for Protein Crystallography. Wu P, Noland C, Ultsch M, Edwards B, Harris D, Mayer R, Harris SF. J Lab Autom 21 97-106 (2016)
  11. Acoustic Methods to Monitor Protein Crystallization and to Detect Protein Crystals in Suspensions of Agarose and Lipidic Cubic Phase. Ericson DL, Yin X, Scalia A, Samara YN, Stearns R, Vlahos H, Ellson R, Sweet RM, Soares AS. J Lab Autom 21 107-114 (2016)
  12. research-article Progress in macromolecular crystallography depends on further miniaturization of crystallization experiments. Gorrec F. Drug Discov Today 19 1505-1507 (2014)
  13. Maintaining Microclimates during Nanoliter Chemical Dispensations Using Custom-Designed Source Plate Lids. Foley BJ, Drozd AM, Bollard MT, Laspina D, Podobedov N, Zeniou N, Rao AS, Andi B, Jackimowicz R, Sweet RM, McSweeney S, Soares AS. J Lab Autom 21 115-124 (2016)
  14. Serial crystallography with multi-stage merging of thousands of images. Soares AS, Yamada Y, Jakoncic J, McSweeney S, Sweet RM, Skinner J, Foadi J, Fuchs MR, Schneider DK, Shi W, Andi B, Andrews LC, Bernstein HJ. Acta Crystallogr F Struct Biol Commun 78 281-288 (2022)


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