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The determinants that govern microtubule assembly from the atomic structure of GTP-tubulin.

Nawrotek A, Knossow M, Gigant B
J Mol Biol 412 35-42 (2011)
Related entries: 3ryf, 3ryh, 3ryi

Cited: 101 times
EuropePMC logo PMID: 21787788

Abstract

Tubulin alternates between a soluble curved structure and a microtubule straight conformation. GTP binding to αβ-tubulin is required for microtubule assembly, but whether this triggers conversion into a straighter structure is still debated. This is due, at least in part, to the lack of structural data for GTP-tubulin before assembly. Here, we report atomic-resolution crystal structures of soluble tubulin in the GDP and GTP nucleotide states in a complex with a stathmin-like domain. The structures differ locally in the neighborhood of the nucleotide. A loop movement in GTP-bound tubulin favors its recruitment to the ends of growing microtubules and facilitates its curved-to-straight transition, but this conversion has not proceeded yet. The data therefore argue for the conformational change toward the straight structure occurring as microtubule-specific contacts are established. They also suggest a model for the way the tubulin structure is modified in relation to microtubule assembly.

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Articles - 3ryc mentioned but not cited (17)

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Reviews citing this publication (18)

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Articles citing this publication (64)

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