› 3rzz

X-ray diffraction
2.2Å resolution

Structure of Hydroxyethylphoshphonate Dioxygenase Y98F Mutant

Released:
DOI: 10.2210/pdb3rzz/pdb
PDB entry 3rzz coloured by chain, front view.
PDB entry 3rzz coloured by chain, side view.
PDB entry 3rzz coloured by chain, top view.
Source organism: Streptomyces viridochromogenes
Primary publication:
Mechanism and substrate recognition of 2-hydroxyethylphosphonate dioxygenase.
Peck SC, Cooke HA, Cicchillo RM, Malova P, Hammerschmidt F, Nair SK, van der Donk WA
Biochemistry 50 6598-605 (2011)
PMID: 21711001

Function and Biology Details

Reaction catalysed: 
2-hydroxyethylphosphonate + O(2) = hydroxymethylphosphonate + formate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177255 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-hydroxyethylphosphonate dioxygenase Chain: A
Molecule details ›
Chain: A
Length: 446 amino acids
Theoretical weight: 48.41 KDa
Source organism: Streptomyces viridochromogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5IW40 (Residues: 1-443; Coverage: 100%)
Gene names: SSQG_01041, hepD, phpD
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CD 15 x CD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: C2221
Unit cell:
a: 84.747Å b: 94.964Å c: 101.396Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.207 0.268
Expression system: Escherichia coli

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Citations

1 review citation

Applications of density functional theory to iron-containing molecules of bioinorganic interest.
Hirao et al. (2014)
5 other articles

1 mention without citation

Mechanism and substrate recognition of 2-hydroxyethylphosphonate dioxygenase.
Peck et al. (2011)