› 3s1t

X-ray diffraction
1.63Å resolution

Structure of the regulatory domain of aspartokinase (Rv3709c; AK-beta) in complex with threonine from Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb3s1t/pdb
PDB entry 3s1t coloured by chain, front view.
PDB entry 3s1t coloured by chain, side view.
PDB entry 3s1t coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Entry authors: Schuldt L, Mueller-Dieckmann J, Weiss MS

Function and Biology Details

Reaction catalysed: 
ATP + L-aspartate = ADP + 4-phospho-L-aspartate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162012 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Aspartokinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 181 amino acids
Theoretical weight: 19.36 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WPX3 (Residues: 251-421; Coverage: 41%)
Gene names: MTV025.057c, Rv3709c, ask
Sequence domains:
Structure domains: Alpha-Beta Plaits
Aspartokinase Chain: C
Molecule details ›
Chain: C
Length: 11 amino acids
Theoretical weight: 1.14 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WPX3 (Residues: 412-421; Coverage: 2%)
Gene names: MTV025.057c, Rv3709c, ask

Ligands and Environments

2 bound ligands:
Ligand THR 2 x THR
Ligand SO4 7 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X12
Spacegroup: P212121
Unit cell:
a: 53.7Å b: 63.43Å c: 108.85Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.193 0.211
Expression system: Escherichia coli BL21(DE3)

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