› 3s56

X-ray diffraction
1.88Å resolution

HIV-1 protease triple mutants V32I, I47V, V82I with antiviral drug saquinavir

Released:
DOI: 10.2210/pdb3s56/pdb
PDB entry 3s56 coloured by chain, front view.
PDB entry 3s56 coloured by chain, side view.
PDB entry 3s56 coloured by chain, top view.
Source organism: Human immunodeficiency virus 1
Primary publication:
Critical differences in HIV-1 and HIV-2 protease specificity for clinical inhibitors.
Tie Y, Wang YF, Boross PI, Chiu TY, Ghosh AK, Tozser J, Louis JM, Harrison RW, Weber IT
Protein Sci 21 339-50 (2012)
PMID: 22238126

Function and Biology Details

Reaction catalysed: 
Specific for a P1 residue that is hydrophobic, and P1' variable, but often Pro.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181991 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidase A2 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 99 amino acids
Theoretical weight: 10.75 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7SSI0 (Residues: 1-99; Coverage: 100%)
Gene name: pol
Sequence domains: Retroviral aspartyl protease
Structure domains: Acid Proteases

Ligands and Environments

2 bound ligands:
Ligand ROC 2 x ROC
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 29.203Å b: 67.416Å c: 92.751Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.186 0.234
Expression system: Escherichia coli

Quick links

Citations

6 review citations

HIV protease inhibitors: a review of molecular selectivity and toxicity.
Lv et al. (2015)
5 more

5 mentions without citation

Critical differences in HIV-1 and HIV-2 protease specificity for clinical inhibitors.
Tie et al. (2012)
4 more