› 3sm3

X-ray diffraction
2.2Å resolution

Crystal Structure of SAM-dependent methyltransferases Q8PUK2_METMA from Methanosarcina mazei. Northeast Structural Genomics Consortium Target MaR262.

Released:
DOI: 10.2210/pdb3sm3/pdb
PDB entry 3sm3 coloured by chain, front view.
PDB entry 3sm3 coloured by chain, side view.
PDB entry 3sm3 coloured by chain, top view.
Source organism: Methanosarcina mazei Go1
Entry authors: Vorobiev S, Neely H, Seetharaman J, Snyder A, Patel P, Xiao R, Ciccosanti C, Everett JK, Nair R, Acton TB, Rost B, Montelione GT, Hunt JF, Tong L, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185806 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methyltransferase type 11 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 235 amino acids
Theoretical weight: 26.97 KDa
Source organism: Methanosarcina mazei Go1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8PUK2 (Residues: 1-227; Coverage: 100%)
Gene name: MM_2332
Sequence domains: Methyltransferase domain
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P4122
Unit cell:
a: 51.589Å b: 51.589Å c: 207.028Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.192 0.246
Expression system: Escherichia coli BL21(DE3)

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