› 3snz

X-ray diffraction
2Å resolution

Crystal structure of a mutant W39D of a betagamma-crystallin domain from Clostridium beijerinckii

Released:
DOI: 10.2210/pdb3snz/pdb
PDB entry 3snz coloured by chain, front view.
PDB entry 3snz coloured by chain, side view.
PDB entry 3snz coloured by chain, top view.
Source organism: Clostridium beijerinckii NCIMB 8052
Primary publication:
Decoding the molecular design principles underlying Ca(2+) binding to βγ-crystallin motifs.
Mishra A, Suman SK, Srivastava SS, Sankaranarayanan R, Sharma Y
J Mol Biol 415 75-91 (2012)
PMID: 22099475

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107978 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta and gamma crystallin Chain: A
Molecule details ›
Chain: A
Length: 97 amino acids
Theoretical weight: 10.62 KDa
Source organism: Clostridium beijerinckii NCIMB 8052
Expression system: Escherichia coli
UniProt:
  • Canonical: A6LX94 (Residues: 118-213; Coverage: 17%)
Gene name: Cbei_2825
Structure domains: Crystallins

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: I432
Unit cell:
a: 110.071Å b: 110.071Å c: 110.071Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.208 0.246
Expression system: Escherichia coli

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Citations

2 review citations

Ca2+-binding motif of βγ-crystallins.
Srivastava et al. (2014)
1 more