› 3spj

X-ray diffraction
3.31Å resolution

Apo inward rectifier potassium channel Kir2.2 I223L mutant

Released:
DOI: 10.2210/pdb3spj/pdb
PDB entry 3spj coloured by chain, front view.
PDB entry 3spj coloured by chain, side view.
PDB entry 3spj coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Structural basis of PIP2 activation of the classical inward rectifier K+ channel Kir2.2.
Hansen SB, Tao X, MacKinnon R
Nature 477 495-8 (2011)
PMID: 21874019

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123320 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-sensitive inward rectifier potassium channel 12 Chain: A
Molecule details ›
Chain: A
Length: 343 amino acids
Theoretical weight: 39.36 KDa
Source organism: Gallus gallus
Expression system: Komagataella pastoris
UniProt:
  • Canonical: F1NHE9 (Residues: 37-372; Coverage: 78%)
Gene name: KCNJ12
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand K 5 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: I4
Unit cell:
a: 83.769Å b: 83.769Å c: 197.126Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.236 0.235 0.267
Expression system: Komagataella pastoris

Quick links

Citations

100 review citations

Phosphoinositides: tiny lipids with giant impact on cell regulation.
Balla T. (2013)
99 more

4 mentions without citation

Structural basis of PIP2 activation of the classical inward rectifier K+ channel Kir2.2.
Hansen et al. (2011)
3 more