› 3ssk

X-ray diffraction
1.36Å resolution

Engineered high-affinity halide-binding protein derived from YFP: bromide complex

Released:
DOI: 10.2210/pdb3ssk/pdb
PDB entry 3ssk coloured by chain, front view.
PDB entry 3ssk coloured by chain, side view.
PDB entry 3ssk coloured by chain, top view.
Source organism: Aequorea victoria
Entry authors: Wang W, Grimley JS, Beese LS, Hellinga HW

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 258 amino acids
Theoretical weight: 29.31 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 2-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand BR 3 x BR
1 modified residue:
Ligand CR2 1 x CR2

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 51.379Å b: 62.361Å c: 69.629Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.132 0.129 0.172
Expression system: Escherichia coli

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