› 3sso

X-ray diffraction
1.9Å resolution

MycE Methyltransferase from the Mycinamycin Biosynthetic Pathway in Complex with Mg and SAH, Crystal form 2

Released:
DOI: 10.2210/pdb3sso/pdb
PDB entry 3sso coloured by chain, front view.
PDB entry 3sso coloured by chain, side view.
PDB entry 3sso coloured by chain, top view.
Source organism: Micromonospora griseorubida
Primary publication:
A new structural form in the SAM/metal-dependent o‑methyltransferase family: MycE from the mycinamicin biosynthetic pathway.
Akey DL, Li S, Konwerski JR, Confer LA, Bernard SM, Anzai Y, Kato F, Sherman DH, Smith JL
J Mol Biol 413 438-50 (2011)
PMID: 21884704

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + mycinamicin VI = S-adenosyl-L-homocysteine + mycinamicin III
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182972 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mycinamicin VI 2''-O-methyltransferase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 419 amino acids
Theoretical weight: 47.04 KDa
Source organism: Micromonospora griseorubida
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q83WF2 (Residues: 1-399; Coverage: 100%)
Gene name: mycE
Sequence domains: MycE methyltransferase N-terminal
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 6 x MG
Ligand SAH 6 x SAH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: C2221
Unit cell:
a: 144.22Å b: 250.846Å c: 158.315Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.145 0.144 0.176
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Architectures, mechanisms and molecular evolution of natural product methyltransferases.
Liscombe et al. (2012)
2 more

1 mention without citation

A new structural form in the SAM/metal-dependent o‑methyltransferase family: MycE from the mycinamicin biosynthetic pathway.
Akey et al. (2011)