› 3szj

X-ray diffraction
1.45Å resolution

Structure of the shwanavidin-biotin complex

Released:
DOI: 10.2210/pdb3szj/pdb
PDB entry 3szj coloured by chain, front view.
PDB entry 3szj coloured by chain, side view.
PDB entry 3szj coloured by chain, top view.
Source organism: Shewanella denitrificans OS217
Primary publication:
Structural adaptation of a thermostable biotin-binding protein in a psychrophilic environment.
Meir A, Bayer EA, Livnah O
J Biol Chem 287 17951-62 (2012)
PMID: 22493427

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171491 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Avidin/streptavidin Chains: A, B
Molecule details ›
Chains: A, B
Length: 122 amino acids
Theoretical weight: 13.04 KDa
Source organism: Shewanella denitrificans OS217
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12QS6 (Residues: 43-162; Coverage: 70%)
Gene name: Sden_0912
Sequence domains: Avidin family
Structure domains: Avidin-like

Ligands and Environments

4 bound ligands:
Ligand BTN 2 x BTN
Ligand GOL 1 x GOL
Ligand CO3 1 x CO3
Ligand ACY 2 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 42.725Å b: 56.031Å c: 86.62Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.162 0.19
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Streptavidin-biotin technology: improvements and innovations in chemical and biological applications.
Dundas et al. (2013)
1 more

4 mentions without citation

Structural adaptation of a thermostable biotin-binding protein in a psychrophilic environment.
Meir et al. (2012)
3 more