› 3t0x

X-ray diffraction
1.96Å resolution

Fluorogen Activating Protein M8VLA4(S55P) in complex with dimethylindole red

Released:
DOI: 10.2210/pdb3t0x/pdb
PDB entry 3t0x coloured by chain, front view.
PDB entry 3t0x coloured by chain, side view.
PDB entry 3t0x coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A variable light domain fluorogen activating protein homodimerizes to activate dimethylindole red.
Senutovitch N, Stanfield RL, Bhattacharyya S, Rule GS, Wilson IA, Armitage BA, Waggoner AS, Berget PB
Biochemistry 51 2471-85 (2012)
PMID: 22390683

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-213401 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Immunoglobulin variable lambda domain M8VLA4(S55P) Chains: A, B
Molecule details ›
Chains: A, B
Length: 123 amino acids
Theoretical weight: 13.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

3 bound ligands:
Ligand DIW 1 x DIW
Ligand EDO 7 x EDO
Ligand SO4 1 x SO4
1 modified residue:
Ligand PCA 2 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P43212
Unit cell:
a: 83.494Å b: 83.494Å c: 76.347Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.209 0.242
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Linkers in the structural biology of protein-protein interactions.
Reddy Chichili et al. (2013)
2 more

1 mention without citation

A variable light domain fluorogen activating protein homodimerizes to activate dimethylindole red.
Senutovitch et al. (2012)