3t77

X-ray diffraction
1.74Å resolution

S25-2- A(2-4)KDO disaccharide complex

Released:
Source organism: Mus musculus
Primary publication:
Germline antibody recognition of distinct carbohydrate epitopes.
Nat Struct Biol 10 1019-25 (2003)
PMID: 14625588

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-560332 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Ig-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 219 amino acids
Theoretical weight: 24.24 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q52L64 (Residues: 21-240; Coverage: 100%)
Gene names: Gm10883, Igkv8-30
Sequence domains:
Structure domains: Immunoglobulins
S25-2 FAB (IGG1K) heavy chain Chain: B
Molecule details ›
Chain: B
Length: 224 amino acids
Theoretical weight: 24.33 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: KDA, KDO
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P212121
Unit cell:
a: 45.9Å b: 81.8Å c: 130.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.206 0.236
Expression system: Escherichia coli