3t9n › Citations

Structure and molecular mechanism of an anion-selective mechanosensitive channel of small conductance.

Zhang X, Wang J, Feng Y, Ge J, Li W, Sun W, Iscla I, Yu J, Blount P, Li Y, Yang M
Proc Natl Acad Sci U S A 109 18180-5 (2012)
Cited: 47 times
EuropePMC logo PMID: 23074248

Abstract

Mechanosensitive (MS) channels are universal cellular membrane pores. Bacterial MS channels, as typified by MS channel of small conductance (MscS) from Escherichia coli (EcMscS), release osmolytes under hypoosmotic conditions. MS channels are known to be ion selective to different extents, but the underlying mechanism remains poorly understood. Here we identify an anion-selective MscS channel from Thermoanaerobacter tengcongensis (TtMscS). The structure of TtMscS closely resembles that of EcMscS, but it lacks the large cytoplasmic equatorial portals found in EcMscS. In contrast, the cytoplasmic pore formed by the C-terminal β-barrel of TtMscS is larger than that of EcMscS and has a strikingly different pattern of electrostatic surface potential. Swapping the β-barrel region between TtMscS and EcMscS partially switches the ion selectivity. Our study defines the role of the β-barrel in the ion selection of an anion-selective MscS channel and provides a structural basis for understanding the ion selectivity of MscS channels.

Articles - 3t9n mentioned but not cited (9)

  1. Open and shut: crystal structures of the dodecylmaltoside solubilized mechanosensitive channel of small conductance from Escherichia coli and Helicobacter pylori at 4.4 Å and 4.1 Å resolutions. Lai JY, Poon YS, Kaiser JT, Rees DC. Protein Sci 22 502-509 (2013)
  2. Structure and molecular mechanism of an anion-selective mechanosensitive channel of small conductance. Zhang X, Wang J, Feng Y, Ge J, Li W, Sun W, Iscla I, Yu J, Blount P, Li Y, Yang M. Proc Natl Acad Sci U S A 109 18180-18185 (2012)
  3. Spatial arrangement of proteins in planar and curved membranes by PPM 3.0. Lomize AL, Todd SC, Pogozheva ID. Protein Sci 31 209-220 (2022)
  4. Saposin Lipid Nanoparticles: A Highly Versatile and Modular Tool for Membrane Protein Research. Flayhan A, Mertens HDT, Ural-Blimke Y, Martinez Molledo M, Svergun DI, Löw C. Structure 26 345-355.e5 (2018)
  5. Probing the structure of the mechanosensitive channel of small conductance in lipid bilayers with pulsed electron-electron double resonance. Ward R, Pliotas C, Branigan E, Hacker C, Rasmussen A, Hagelueken G, Booth IR, Miller S, Lucocq J, Naismith JH, Schiemann O. Biophys J 106 834-842 (2014)
  6. A novel mechanosensitive channel controls osmoregulation, differentiation, and infectivity in Trypanosoma cruzi. Dave N, Cetiner U, Arroyo D, Fonbuena J, Tiwari M, Barrera P, Lander N, Anishkin A, Sukharev S, Jimenez V. Elife 10 e67449 (2021)
  7. Selectivity mechanisms in MscS-like channels: From structure to function. Cox CD, Wann KT, Martinac B. Channels (Austin) 8 5-12 (2014)
  8. An automated data processing and analysis pipeline for transmembrane proteins in detergent solutions. Molodenskiy DS, Mertens HDT, Svergun DI. Sci Rep 10 8081 (2020)
  9. ProtCHOIR: a tool for proteome-scale generation of homo-oligomers. Torres PHM, Rossi AD, Blundell TL. Brief Bioinform 22 bbab182 (2021)


Reviews citing this publication (12)

  1. United in diversity: mechanosensitive ion channels in plants. Hamilton ES, Schlegel AM, Haswell ES. Annu Rev Plant Biol 66 113-137 (2015)
  2. MscS-like mechanosensitive channels in plants and microbes. Wilson ME, Maksaev G, Haswell ES. Biochemistry 52 5708-5722 (2013)
  3. Life with Bacterial Mechanosensitive Channels, from Discovery to Physiology to Pharmacological Target. Blount P, Iscla I. Microbiol Mol Biol Rev 84 e00055-19 (2020)
  4. The evolutionary 'tinkering' of MscS-like channels: generation of structural and functional diversity. Cox CD, Nakayama Y, Nomura T, Martinac B. Pflugers Arch 467 3-13 (2015)
  5. The evolution of bacterial mechanosensitive channels. Booth IR, Miller S, Müller A, Lehtovirta-Morley L. Cell Calcium 57 140-150 (2015)
  6. Spectator no more, the role of the membrane in regulating ion channel function. Pliotas C, Naismith JH. Curr Opin Struct Biol 45 59-66 (2017)
  7. Bacterial mechanosensitive channels: models for studying mechanosensory transduction. Martinac B, Nomura T, Chi G, Petrov E, Rohde PR, Battle AR, Foo A, Constantine M, Rothnagel R, Carne S, Deplazes E, Cornell B, Cranfield CG, Hankamer B, Landsberg MJ. Antioxid Redox Signal 20 952-969 (2014)
  8. From membrane tension to channel gating: A principal energy transfer mechanism for mechanosensitive channels. Zhang XC, Liu Z, Li J. Protein Sci 25 1954-1964 (2016)
  9. Ultrasonic Neuromodulation and Sonogenetics: A New Era for Neural Modulation. Wang S, Meng W, Ren Z, Li B, Zhu T, Chen H, Wang Z, He B, Zhao D, Jiang H. Front Physiol 11 787 (2020)
  10. Organellar mechanosensitive channels involved in hypo-osmoregulation in fission yeast. Nakayama Y, Iida H. Cell Calcium 56 467-471 (2014)
  11. Roles of Bacterial Mechanosensitive Channels in Infection and Antibiotic Susceptibility. Sidarta M, Baruah L, Wenzel M. Pharmaceuticals (Basel) 15 770 (2022)
  12. How Functional Lipids Affect the Structure and Gating of Mechanosensitive MscS-like Channels. Flegler VJ, Rasmussen T, Böttcher B. Int J Mol Sci 23 15071 (2022)

Articles citing this publication (26)



Quick links