› 3ta4

X-ray diffraction
2.35Å resolution

Small laccase from Amycolatopsis sp. ATCC 39116 complexed with 1-(3,4-dimethoxyphenyl)-2-(2-methoxyphenoxy)-1,3-dihydroxypropane

Released:
DOI: 10.2210/pdb3ta4/pdb
PDB entry 3ta4 coloured by chain, front view.
PDB entry 3ta4 coloured by chain, side view.
PDB entry 3ta4 coloured by chain, top view.
Source organism: Amycolatopsis sp. ATCC 39116
Primary publication:
Roles of small laccases from Streptomyces in lignin degradation.
Majumdar S, Lukk T, Solbiati JO, Bauer S, Nair SK, Cronan JE, Gerlt JA
Biochemistry 53 4047-58 (2014)
PMID: 24870309

Function and Biology Details

Reaction catalysed: 
4 benzenediol + O(2) = 4 benzosemiquinone + 2 H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-516359 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Small laccase, multi-copper oxidase Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 299 amino acids
Theoretical weight: 33.25 KDa
Source organism: Amycolatopsis sp. ATCC 39116
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: J9PBQ8 (Residues: 1-279; Coverage: 100%)
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

2 bound ligands:
Ligand CU 16 x CU
Ligand TO2 1 x TO2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: C2221
Unit cell:
a: 84.42Å b: 115.26Å c: 163.51Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.176 0.238
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

19 review citations

Lignocellulose degradation mechanisms across the Tree of Life.
Cragg et al. (2015)
18 more

1 mention without citation

Secretory expression of recombinant small laccase genes in Gram-positive bacteria.
Välimets et al. (2023)