› 3tdx

X-ray diffraction
2.5Å resolution

Crystal structure of HSC L82V

Released:
DOI: 10.2210/pdb3tdx/pdb
PDB entry 3tdx coloured by chain, front view.
PDB entry 3tdx coloured by chain, side view.
PDB entry 3tdx coloured by chain, top view.
Source organism: Clostridioides difficile 630
Primary publication:
Identification and characterization of a bacterial hydrosulphide ion channel.
Czyzewski BK, Wang DN
Nature 483 494-7 (2012)
PMID: 22407320

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-557115 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Formate/nitrite transporter Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 268 amino acids
Theoretical weight: 28.25 KDa
Source organism: Clostridioides difficile 630
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q186B7 (Residues: 2-258; Coverage: 100%)
Gene name: CD630_22300
Sequence domains: Formate/nitrite transporter
Structure domains: Glycerol uptake facilitator protein.

Ligands and Environments

3 bound ligands:
Ligand PG4 5 x PG4
Ligand CL 5 x CL
Ligand BOG 2 x BOG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 99.012Å b: 118.661Å c: 151.343Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.162 0.208
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

18 review citations

H₂S signalling through protein sulfhydration and beyond.
Paul et al. (2012)
17 more

3 mentions without citation

C-H⋯S hydrogen bonding interactions.
Fargher et al. (2022)
2 more