› 3tia

X-ray diffraction
1.8Å resolution

Crystal structure of 1957 pandemic H2N2 neuraminidase complexed with laninamivir

Released:
DOI: 10.2210/pdb3tia/pdb
PDB entry 3tia coloured by chain, front view.
PDB entry 3tia coloured by chain, side view.
PDB entry 3tia coloured by chain, top view.
Source organism: Influenza A virus (A/RI/5+/1957(H2N2))
Primary publication:
Structural and functional analysis of laninamivir and its octanoate prodrug reveals group specific mechanisms for influenza NA inhibition.
Vavricka CJ, Li Q, Wu Y, Qi J, Wang M, Liu Y, Gao F, Liu J, Feng E, He J, Wang J, Liu H, Jiang H, Gao GF
OpenAccess logo PLoS Pathog 7 e1002249 (2011)
PMID: 22028647

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172754 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Neuraminidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 469 amino acids
Theoretical weight: 52.07 KDa
Source organism: Influenza A virus (A/RI/5+/1957(H2N2))
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q194T1 (Residues: 1-469; Coverage: 100%)
Gene name: NA
Sequence domains: Neuraminidase
Structure domains: Neuraminidase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
3 bound ligands:
Ligand CA 4 x CA
Ligand LNV 4 x LNV
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: P21
Unit cell:
a: 89.928Å b: 140.305Å c: 89.893Å
α: 90° β: 101.54° γ: 90°
R-values:
R R work R free
0.152 0.152 0.17
Expression system: Spodoptera frugiperda

Quick links

Citations

13 review citations

Influenza virus resistance to neuraminidase inhibitors.
Samson et al. (2013)
12 more

9 mentions without citation

Structural and functional analysis of laninamivir and its octanoate prodrug reveals group specific mechanisms for influenza NA inhibition.
Vavricka et al. (2011)
8 more