› 3tp2

X-ray diffraction
2.4Å resolution

Crystal Structure of the Splicing Factor Cwc2 from yeast

Released:
DOI: 10.2210/pdb3tp2/pdb
PDB entry 3tp2 coloured by chain, front view.
PDB entry 3tp2 coloured by chain, side view.
PDB entry 3tp2 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Crystal structure of Cwc2 reveals a novel architecture of a multipartite RNA-binding protein.
Schmitzová J, Rasche N, Dybkov O, Kramer K, Fabrizio P, Urlaub H, Lührmann R, Pena V
EMBO J 31 2222-34 (2012)
PMID: 22407296

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171282 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pre-mRNA-splicing factor CWC2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 229 amino acids
Theoretical weight: 26.02 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12046 (Residues: 1-227; Coverage: 67%)
Gene names: CWC2, D1041, NTC40, SLC3, YDL209C
Sequence domains:
Structure domains: Alpha-Beta Plaits

Ligands and Environments

2 bound ligands:
Ligand NA 2 x NA
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 58.7Å b: 79.7Å c: 113.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.221 0.256
Expression system: Escherichia coli

Quick links

Citations

2 review citations

CryoEM structures of two spliceosomal complexes: starter and dessert at the spliceosome feast.
Nguyen et al. (2016)
1 more

4 mentions without citation

Structural studies of the spliceosome: zooming into the heart of the machine.
Galej et al. (2014)
3 more