› 3tts

X-ray diffraction
2.4Å resolution

Crystal structure of beta-galactosidase from Bacillus circulans sp. alkalophilus

Released:
DOI: 10.2210/pdb3tts/pdb
PDB entry 3tts coloured by chain, front view.
PDB entry 3tts coloured by chain, side view.
PDB entry 3tts coloured by chain, top view.
Source organism: Niallia circulans subsp. alkalophilus
Primary publication:
Structural analysis, enzymatic characterization, and catalytic mechanisms of β-galactosidase from Bacillus circulans sp. alkalophilus.
Maksimainen M, Paavilainen S, Hakulinen N, Rouvinen J
FEBS J 279 1788-98 (2012)
PMID: 22385475

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
homo trimer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-164087 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-galactosidase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 675 amino acids
Theoretical weight: 77.41 KDa
Source organism: Niallia circulans subsp. alkalophilus

Ligands and Environments

1 bound ligand:
Ligand ZN 6 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: R3
Unit cell:
a: 226Å b: 226Å c: 246.4Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.155 0.152 0.206

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Citations

4 review citations

Cold-Active β-Galactosidases: Insight into Cold Adaption Mechanisms and Biotechnological Exploitation.
Mangiagalli et al. (2021)
3 more

7 mentions without citation

Structural insights into the substrate specificity of Streptococcus pneumoniae β(1,3)-galactosidase BgaC.
Cheng et al. (2012)
6 more