› 3tui

X-ray diffraction
2.9Å resolution

Inward facing conformations of the MetNI methionine ABC transporter: CY5 native crystal form

Released:
DOI: 10.2210/pdb3tui/pdb
PDB entry 3tui coloured by chain, front view.
PDB entry 3tui coloured by chain, side view.
PDB entry 3tui coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Inward facing conformations of the MetNI methionine ABC transporter: Implications for the mechanism of transinhibition.
Johnson E, Nguyen PT, Yeates TO, Rees DC
Protein Sci 21 84-96 (2012)
PMID: 22095702

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + D-methionine-[methionine-binding protein](Side 1) = ADP + phosphate + D-methionine(Side 2) + [methionine-binding protein](Side 1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151799 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
D-methionine transport system permease protein MetI Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 217 amino acids
Theoretical weight: 23.27 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P31547 (Residues: 1-217; Coverage: 100%)
Gene names: JW0194, b0198, metI, yaeE
Sequence domains: Binding-protein-dependent transport system inner membrane component
Structure domains: MetI-like
Methionine import ATP-binding protein MetN Chains: C, D, G, H
Molecule details ›
Chains: C, D, G, H
Length: 366 amino acids
Theoretical weight: 40.61 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P30750 (Residues: 1-343; Coverage: 100%)
Gene names: JW0195, abc, b0199, metN
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ADP 4 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P21
Unit cell:
a: 83.43Å b: 140.08Å c: 150.07Å
α: 90° β: 96.39° γ: 90°
R-values:
R R work R free
0.244 0.24 0.303
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

Structure and mechanism of ATP-dependent phospholipid transporters.
López-Marqués et al. (2015)
2 more

7 mentions without citation

Structural diversity of ABC transporters.
ter Beek et al. (2014)
6 more