› 3two

X-ray diffraction
2.18Å resolution

The crystal structure of CAD from Helicobacter pylori complexed with NADP(H)

Released:
DOI: 10.2210/pdb3two/pdb
PDB entry 3two coloured by chain, front view.
PDB entry 3two coloured by chain, side view.
PDB entry 3two coloured by chain, top view.
Source organism: Helicobacter pylori 51
Primary publication:
Unusual NADPH conformation in the crystal structure of a cinnamyl alcohol dehydrogenase from Helicobacter pylori in complex with NADP(H) and substrate docking analysis.
Seo KH, Zhuang N, Chen C, Song JY, Kang HL, Rhee KH, Lee KH
FEBS Lett 586 337-43 (2012)
PMID: 22269576

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-241343 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mannitol dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 348 amino acids
Theoretical weight: 38.74 KDa
Source organism: Helicobacter pylori 51
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand NDP 2 x NDP
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 6C1
Spacegroup: P212121
Unit cell:
a: 85.41Å b: 85.68Å c: 100.491Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.173 0.226
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Activity prediction of substrates in NADH-dependent carbonyl reductase by docking requires catalytic constraints and charge parameterization of catalytic zinc environment.
Dhoke et al. (2015)
1 more

2 mentions without citation

Structure of Streptococcus agalactiae tip pilin GBS104: a model for GBS pili assembly and host interactions.
Krishnan et al. (2013)
1 more