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Structure and function of the small terminase component of the DNA packaging machine in T4-like bacteriophages.

Sun S, Gao S, Kondabagil K, Xiang Y, Rossmann MG, Rao VB
Proc Natl Acad Sci U S A 109 817-22 (2012)
Cited: 63 times
EuropePMC logo PMID: 22207623

Abstract

Tailed DNA bacteriophages assemble empty procapsids that are subsequently filled with the viral genome by means of a DNA packaging machine situated at a special fivefold vertex. The packaging machine consists of a "small terminase" and a "large terminase" component. One of the functions of the small terminase is to initiate packaging of the viral genome, whereas the large terminase is responsible for the ATP-powered translocation of DNA. The small terminase subunit has three domains, an N-terminal DNA-binding domain, a central oligomerization domain, and a C-terminal domain for interacting with the large terminase. Here we report structures of the central domain in two different oligomerization states for a small terminase from the T4 family of phages. In addition, we report biochemical studies that establish the function for each of the small terminase domains. On the basis of the structural and biochemical information, we propose a model for DNA packaging initiation.

Reviews - 3txs mentioned but not cited (2)

  1. Viral Small Terminase: A Divergent Structural Framework for a Conserved Biological Function. Lokareddy RK, Hou CD, Li F, Yang R, Cingolani G. Viruses 14 2215 (2022)
  2. Bacteriophage T4 Head: Structure, Assembly, and Genome Packaging. Rao VB, Fokine A, Fang Q, Shao Q. Viruses 15 527 (2023)

Articles - 3txs mentioned but not cited (3)

  1. Structure and function of the small terminase component of the DNA packaging machine in T4-like bacteriophages. Sun S, Gao S, Kondabagil K, Xiang Y, Rossmann MG, Rao VB. Proc Natl Acad Sci U S A 109 817-822 (2012)
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  3. Identification of the First Gene Transfer Agent (GTA) Small Terminase in Rhodobacter capsulatus and Its Role in GTA Production and Packaging of DNA. Sherlock D, Leong JX, Fogg PCM. J Virol 93 e01328-19 (2019)


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  15. Architecture of the Complex Formed by Large and Small Terminase Subunits from Bacteriophage P22. McNulty R, Lokareddy RK, Roy A, Yang Y, Lander GC, Heck AJR, Johnson JE, Cingolani G. J Mol Biol 427 3285-3299 (2015)
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  21. Divergent Evolution of Nuclear Localization Signal Sequences in Herpesvirus Terminase Subunits. Sankhala RS, Lokareddy RK, Cingolani G. J Biol Chem 291 11420-11433 (2016)
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