› 3utg

X-ray diffraction
2.25Å resolution

Crystal structure of Aspergillus fumigatus UDP galactopyranose mutase complexed with UDP in reduced state

Released:
DOI: 10.2210/pdb3utg/pdb
PDB entry 3utg coloured by chain, front view.
PDB entry 3utg coloured by chain, side view.
PDB entry 3utg coloured by chain, top view.
Source organism: Aspergillus fumigatus
Primary publication:
Crystal structures and small-angle x-ray scattering analysis of UDP-galactopyranose mutase from the pathogenic fungus Aspergillus fumigatus.
Dhatwalia R, Singh H, Oppenheimer M, Karr DB, Nix JC, Sobrado P, Tanner JJ
J Biol Chem 287 9041-51 (2012)
PMID: 22294687

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-galactopyranose = UDP-alpha-D-galactofuranose
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175786 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-galactopyranose mutase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 513 amino acids
Theoretical weight: 57.13 KDa
Source organism: Aspergillus fumigatus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4W1X2 (Residues: 1-510; Coverage: 100%)
Gene name: glfA
Sequence domains: NAD(P)-binding Rossmann-like domain
Structure domains: FAD/NAD(P)-binding domain

Ligands and Environments


Cofactor: Ligand FDA 4 x FDA
2 bound ligands:
Ligand SO4 18 x SO4
Ligand UDP 2 x UDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P6522
Unit cell:
a: 218.176Å b: 218.176Å c: 320.822Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.193 0.192 0.218
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Determination of protein oligomeric structure from small-angle X-ray scattering.
Korasick et al. (2018)
3 more

6 mentions without citation

Structure, mechanism, and dynamics of UDP-galactopyranose mutase.
Tanner et al. (2014)
5 more