› 3vey

X-ray diffraction
2.25Å resolution

glucokinase in complex with glucose and ATPgS

Released:
DOI: 10.2210/pdb3vey/pdb
PDB entry 3vey coloured by chain, front view.
PDB entry 3vey coloured by chain, side view.
PDB entry 3vey coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Insights into mechanism of glucokinase activation: observation of multiple distinct protein conformations.
Liu S, Ammirati MJ, Song X, Knafels JD, Zhang J, Greasley SE, Pfefferkorn JA, Qiu X
J Biol Chem 287 13598-610 (2012)
PMID: 22298776

Function and Biology Details

Reaction catalysed: 
ATP + D-hexose = ADP + D-hexose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152941 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hexokinase-4 Chain: A
Molecule details ›
Chain: A
Length: 455 amino acids
Theoretical weight: 51.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P35557 (Residues: 16-465; Coverage: 97%)
  • Best match: P35557-2 (Residues: 17-466)
Gene name: GCK
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand GLC 1 x GLC
Ligand NA 1 x NA
Ligand AGS 1 x AGS
Ligand 0H5 1 x 0H5
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P6522
Unit cell:
a: 80.387Å b: 80.387Å c: 324.334Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.216 0.214 0.246
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Solution NMR Spectroscopy for the Study of Enzyme Allostery.
Lisi et al. (2016)
10 more

4 mentions without citation

Insights into mechanism of glucokinase activation: observation of multiple distinct protein conformations.
Liu et al. (2012)
3 more