› 3vfc

X-ray diffraction
2Å resolution

Crystal structure of enolase MSMEG_6132 (TARGET EFI-502282) from Mycobacterium smegmatis str. MC2 155 complexed with tartrate

Released:
DOI: 10.2210/pdb3vfc/pdb
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PDB entry 3vfc coloured by chain, front view.
PDB entry 3vfc coloured by chain, side view.
PDB entry 3vfc coloured by chain, top view.
Source organism: Mycolicibacterium smegmatis MC2 155
Entry authors: Patskovsky Y, Toro R, Bhosle R, Hillerich B, Seidel RD, Washington E, Scott Glenn A, Chowdhury S, Evans B, Hammonds J, Zencheck WD, Imker HJ, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Reaction catalysed: 
D-glucarate = 5-dehydro-4-deoxy-D-glucarate + H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106466 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
glucarate dehydratase Chain: A
Molecule details ›
Chain: A
Length: 445 amino acids
Theoretical weight: 47.42 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0R5B5 (Residues: 1-423; Coverage: 100%)
Gene name: MSMEG_6132
Sequence domains: Enolase C-terminal domain-like
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand TLA 1 x TLA
Ligand DTT 1 x DTT
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P6122
Unit cell:
a: 95.264Å b: 95.264Å c: 260.649Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.161 0.16 0.192
Expression system: Escherichia coli BL21(DE3)

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