3vg3 › Citations

Utility of anion and cation combinations for phasing of protein structures.

Sharma A, Yogavel M, Sharma A
J Struct Funct Genomics 13 135-43 (2012)
Related entries: 3b2h, 3b2i, 3b2j, 3b2k, 3b2l, 3vg2, 3vg4, 3vg5, 3vg6, 3vg7

Cited: 4 times
EuropePMC logo PMID: 22562242

Abstract

We report the use of anionic (I(-)), cationic (Ba(2+), Cd(2+)) and ionic mixtures (I(-) plus Ba(2+)) for derivatizing liver fatty acid binding protein (LFABP) crystals. Use of cationic and anionic salts in phasing experiments revealed distinct non-overlapping sites for these ions, suggesting exclusive binding regions on LFABP. Interestingly, cations of identical charge and valency (like Ba(2+) and Cd(2+)) bound to distinct pockets on the protein surface. Furthermore, a mixture of salts containing both I(-) and Ba(2+) was very useful in phasing experiments as these oppositely charged ions bound to different regions of LFABP. Our data therefore suggest that cationic and anionic salt mixtures like BaCl(2) with NH(4)I or salts like CdI, BaI where each ion has a significant anomalous signal for a given X-ray wavelength may be valuable reagents for phasing during structure determination.

Reviews citing this publication (1)

  1. L-FABP: A novel biomarker of kidney disease. Xu Y, Xie Y, Shao X, Ni Z, Mou S. Clin Chim Acta 445 85-90 (2015)

Articles citing this publication (3)

  1. Molecular mechanisms of Charcot-Marie-Tooth neuropathy linked to mutations in human myelin protein P2. Ruskamo S, Nieminen T, Kristiansen CK, Vatne GH, Baumann A, Hallin EI, Raasakka A, Joensuu P, Bergmann U, Vattulainen I, Kursula P. Sci Rep 7 6510 (2017)
  2. Mass spectrometry and NMR analysis of ligand binding by human liver fatty acid binding protein. Santambrogio C, Favretto F, D'Onofrio M, Assfalg M, Grandori R, Molinari H. J Mass Spectrom 48 895-903 (2013)
  3. A Simple Method for On-Gel Detection of Myrosinase Activity. Gonda S, Szűcs Z, Plaszkó T, Cziáky Z, Kiss-Szikszai A, Vasas G, M-Hamvas M. Molecules 23 E2204 (2018)


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