› 3vu2

X-ray diffraction
2.23Å resolution

Structure of the Starch Branching Enzyme I (BEI) complexed with maltopentaose from Oryza sativa L

Released:
DOI: 10.2210/pdb3vu2/pdb
PDB entry 3vu2 coloured by chain, front view.
PDB entry 3vu2 coloured by chain, side view.
PDB entry 3vu2 coloured by chain, top view.
Source organism: Oryza sativa Japonica Group
Primary publication:
Crystal structure of the rice branching enzyme I (BEI) in complex with maltopentaose.
Chaen K, Noguchi J, Omori T, Kakuta Y, Kimura M
Biochem Biophys Res Commun 424 508-11 (2012)
PMID: 22771800

Function and Biology Details

Reaction catalysed: 
Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-554093 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
1,4-alpha-glucan-branching enzyme, chloroplastic/amyloplastic Chains: A, B
Molecule details ›
Chains: A, B
Length: 702 amino acids
Theoretical weight: 81.2 KDa
Source organism: Oryza sativa Japonica Group
Expression system: Escherichia coli
UniProt:
  • Canonical: Q01401 (Residues: 66-767; Coverage: 86%)
Gene names: LOC_Os06g51084, Os06g0726400, P0017G10.8-1, P0017G10.8-2, P0548E04.28-1, P0548E04.28-2, RBE1, SBE1
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
2 bound ligands:
Ligand BGC 4 x BGC
Ligand GOL 5 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P21
Unit cell:
a: 73.81Å b: 66.56Å c: 169.625Å
α: 90° β: 89.97° γ: 90°
R-values:
R R work R free
0.229 0.226 0.286
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Structure and function of α-glucan debranching enzymes.
Møller et al. (2016)
2 more

4 mentions without citation

Functional Roles of Starch Binding Domains and Surface Binding Sites in Enzymes Involved in Starch Biosynthesis.
Wilkens et al. (2018)
3 more