› 3vvb

X-ray diffraction
2.8Å resolution

Crystal Structure of Capsular Polysaccharide Synthesizing Enzyme CapE from Staphylococcus aureus in apo form

Released:
DOI: 10.2210/pdb3vvb/pdb
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Source organism: Staphylococcus aureus subsp. aureus Mu50
Primary publication:
Crystal structure of the capsular polysaccharide synthesizing protein CapE of Staphylococcus aureus.
Miyafusa T, Caaveiro JM, Tanaka Y, Tanner ME, Tsumoto K
OpenAccess logo Biosci Rep 33 (2013)
PMID: 23611437

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-glucose = UDP-alpha-D-galactose
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101926 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-glucose 4-epimerase Chain: A
Molecule details ›
Chain: A
Length: 363 amino acids
Theoretical weight: 40.96 KDa
Source organism: Staphylococcus aureus subsp. aureus Mu50
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H3JPH0 (Residues: 2-342; Coverage: 100%)
Gene names: SAV0153, capE
Sequence domains:
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAP 1 x NAP
No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P6322
Unit cell:
a: 125.18Å b: 125.18Å c: 101.79Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.238 0.236 0.276
Expression system: Escherichia coli

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Citations

10 citation in other articles

USA300 and USA500 clonal lineages of Staphylococcus aureus do not produce a capsular polysaccharide due to conserved mutations in the cap5 locus.
Boyle-Vavra et al. (2015)
9 more

3 mentions without citation

Crystal structure of the capsular polysaccharide synthesizing protein CapE of Staphylococcus aureus.
Miyafusa et al. (2013)
2 more

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