› 3vx4

X-ray diffraction
2.69Å resolution

Crystal Structure of the Nucleotide-Binding Domain of S. mutans ComA, a Bifunctional ATP-binding Cassette Transporter Involved in the Quorum-sensing Pathway

Released:
DOI: 10.2210/pdb3vx4/pdb
PDB entry 3vx4 coloured by chain, front view.
PDB entry 3vx4 coloured by chain, side view.
PDB entry 3vx4 coloured by chain, top view.
Source organism: Streptococcus mutans UA159
Primary publication:
Boundary of the nucleotide-binding domain of Streptococcus ComA based on functional and structural analysis.
Ishii S, Yano T, Okamoto A, Murakawa T, Hayashi H
Biochemistry 52 2545-55 (2013)
PMID: 23534432

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184251 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ABC transporter, ATP-binding protein ComA Chains: A, D
Molecule details ›
Chains: A, D
Length: 273 amino acids
Theoretical weight: 30.35 KDa
Source organism: Streptococcus mutans UA159
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8DW05 (Residues: 495-760; Coverage: 35%)
Gene name: SMU_286
Sequence domains: ABC transporter
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand ATP 2 x ATP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P62
Unit cell:
a: 129.611Å b: 129.611Å c: 72.425Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.194 0.191 0.248
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Plausible Drug Targets in the Streptococcus mutans Quorum Sensing Pathways to Combat Dental Biofilms and Associated Risks.
Kaur et al. (2015)
1 more

3 mentions without citation

Multidrug resistance-associated protein 4 is a bile transporter of Clonorchis sinensis simulated by in silico docking.
Dai et al. (2017)
2 more