› 3w1c

X-ray diffraction
1.3Å resolution

Structure of a pressure sensitive YFP variant YFP-G1

Released:
DOI: 10.2210/pdb3w1c/pdb
PDB entry 3w1c coloured by chain, front view.
PDB entry 3w1c coloured by chain, side view.
PDB entry 3w1c coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Glycine insertion makes yellow fluorescent protein sensitive to hydrostatic pressure.
Watanabe TM, Imada K, Yoshizawa K, Nishiyama M, Kato C, Abe F, Morikawa TJ, Kinoshita M, Fujita H, Yanagida T
OpenAccess logo PLoS One 8 e73212 (2013)
PMID: 24014139

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 237 amino acids
Theoretical weight: 26.93 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 2-144, 145-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CR2 1 x CR2

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P212121
Unit cell:
a: 51.19Å b: 62.76Å c: 69.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.196 0.211
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Measuring forces and stresses in situ in living tissues.
Sugimura et al. (2016)
4 more

1 mention without citation

The Enterobacterium Trabulsiella odontotermitis Presents Novel Adaptations Related to Its Association with Fungus-Growing Termites.
Sapountzis et al. (2015)