› 3w8q

X-ray diffraction
2.2Å resolution

Structure of the Human Mitogen-Activated Protein Kinase Kinase 1 (MEK1)

Released:
DOI: 10.2210/pdb3w8q/pdb
PDB entry 3w8q coloured by chain, front view.
PDB entry 3w8q coloured by chain, side view.
PDB entry 3w8q coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of mitogen-activated protein kinase kinase 1 in the DFG-out conformation.
Nakae S, Kitamura M, Fujiwara D, Sawa M, Shirai T, Fujii I, Tada T
Acta Crystallogr F Struct Biol Commun 77 459-464 (2021)
PMID: 34866601

Function and Biology Details

Reaction catalysed: 
ATP + L-seryl/L-threonyl/L-tyrosyl-[protein] = ADP + O-phospho-L-seryl/O-phospho-L-threonyl/O-phospho-L-tyrosyl-[protein]
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169745 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dual specificity mitogen-activated protein kinase kinase 1 Chain: A
Molecule details ›
Chain: A
Length: 350 amino acids
Theoretical weight: 39.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02750 (Residues: 39-382; Coverage: 88%)
Gene names: MAP2K1, MEK1, PRKMK1
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand AGS 1 x AGS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: C2221
Unit cell:
a: 47.815Å b: 96.191Å c: 138.617Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.198 0.268
Expression system: Escherichia coli

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Citations

1 citations in other articles

Pseudokinase NRP1 facilitates endocytosis of transferrin in the African trypanosome.
Kumar et al. (2022)

17 mentions without citation

Protein Kinase Targets in Breast Cancer.
García-Aranda et al. (2017)
16 more